Murine protein expressed in HEK293 cells used for aspirations in mouse model ?

I am planning to express a murine protein X with a Myc tag in HEK293F cell line - purify the protein - check the purity of the protein - use that protein - X with Myc tag to aspirate mouse. Thus,...

12 February 2020 4,483 1 View

How to get an Asymptote from multiple coordinates obtained from Machine Learning ?

I want know about an algorithm or formula to find the asymptote from coordinates obtained from Machine Learning. Like the ML will always give points precise & closer to the Asymptote if I ran it,...

11 October 2019 1,025 2 View

Any open-access to Hydrology Sandbox Experiment data?

I am working on numeric hydrology model deveolopment with Finite Element Method. I am thinking to test the model with the laboratory Hydrology Sandbox Experiment Data to validate the model. So I...

08 April 2019 2,330 2 View

I have completed my bachelor's in Electrical Engineering and I am looking forward to start learning more about smart grid .From where should I start.?

I want to learn about smart grid and simulating a smart grid network and also the data analytics used in smart grid network .

23 May 2018 6,165 3 View

Does anyone have experience working with Injectable Cyanocobalamin in rats?

15 September 2014 1,045 4 View

When I tried to precipitate my protein with acetone or TCA, it did not precipitate. But when I mix it with water it precipitated - any thoughts?

My protein is acid extracted. I need to know the mechanism behind it.

29 May 2014 6,787 3 View

Free software to conduct an online quiz?

From the last few months, I am trying to generate an online Quiz for my student, I have prepared some of them also, but find two difficulties: 1. Some students can't open the exam in their...

24 December 2013 2,307 3 View

Shortcut key to merge some cells in table & to split the same!

Can anybody suggest the shortcut keys for Merging & splitting the cell in MS word & MS Excel?

05 October 2013 9,215 13 View

What is the ideal dilution for using the Primary and Secondary Antibody Concentration for 6x HIS tag : Protein?

The 1:2000 for primary and 1:20000 for secondary gives a very dark background .

22 May 2013 4,813 3 View

What if the restriction digest pattern of plasmid midiprep varies from the same plasmid's miniprep?

I normally inoculate 5 - 7 colonies from selective antibiotic LB plate after transformation in the liquid LB with the same concentration of the same antibiotic. The next day, I do plasmid miniprep...

27 February 2013 4,896 12 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

How can I obtain time series data by country on night-time light (brightness) ?

Hi everyone, Please, how can I obtain time series data by country on night-time light (brightness) ? I've tried, but so far all I can find are maps. I am looking for numerical data, especially in...

24 February 2021 8,625 4 View

What are difference between laser diodes, simple light emitting diodes and power light emitting diodes when operated in the visible spectrum ?

I want to illuminate a scene (small space) using LED to capture photographs or videos of some tiny objects inside the space. But am not sure if I should use a Laser LED, or simple LED or power...

22 February 2021 4,240 4 View

How to interpret this FTIR spectrum of pyrolyzed hydrochar?

20 February 2021 2,840 3 View

No title

The regenerated cellulose film was prepared by zinc chloride/H2O system. The zinc chloride had been washed away in the regeneration process, and the wet film which was not dried had uniform...

18 February 2021 9,538 1 View

Is nanotechnology part of materials science? Is it possible to obtain Pico technology and move from hypothesis to application?

Pico-scale This is three orders of magnitude smaller than a nanometer (and thus most nanotechnology) and two orders of magnitude smaller than most chemistry transformations and measurements. Pico...

18 February 2021 4,540 5 View

How do I plot Pressure against crank angle degree in Fluent?

18 February 2021 8,390 3 View

How to get rough estimation of the concentrations of Oxygen and moisture inside the glove box?

Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...

17 February 2021 1,012 3 View