I got comments for my manuscript: For migration assay (checking cell-cell interactions), the authors only used transwell. More approaches should be included.
Can I ask does anybody know other methods for migration assay (not transwell based)?
The wounding model can be modified to examine the interaction of 2 cell types. In the simplest case, a barrier is placed in a dish and different cell types seeded on each side. When growth is establishe, the barrier is removed. Patterned substrates are a more sophisticated variation of this theme.
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