I have recently read a publication which describes the last enzyme in the pathway but I need information regarding enzymes which function in earlier steps.
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
Hi, I am working with Invitrogen Seamless cloning which requires 200 ng/ul insert DNA fragment. Protocol recommends to use restriction enzyme to digest pre-cloned plasmid and then elute specific...
25 June 2024 971 2 View
I am carrying out a research project in which I am conducting focus groups with older men and women to study the musical stereotypes learned in traditional Spanish oral music groups, but I am...
10 May 2024 3,852 3 View
We are an R&D laboratory focused on anionic, cationic, and zwitterionic surfactants and usually tackle surfactant synthesis and analysis of products from customers. In particular, we deal with...
29 April 2024 8,927 1 View
We are isolating total RNA from mouse sceletal muscle, using the beads beater with the sample already being in Trizol, afterwards chloroform extraction and finally Qiagen RNeasy kit. On the TAPE...
29 April 2024 3,952 5 View
I’m looking for an inclusive leadership scale to include in a mediation analysis with psych safety and workplace belonging, what would you use
27 April 2024 6,072 2 View
Greetings, I am trying to set up an alkaline Comet Assay. I have no previous experience with this type of assay, unfortunately, so I am doing a lot of trial and error. After embedding my cells in...
23 April 2024 6,534 0 View
This question is directed on the performance of the Sysmex DI-60 digital morphology analyzer and its contributions on the improvement in the laboratory workflow. I am curious as to what...
10 April 2024 3,069 1 View
It is known that self-medication with antibiotics is an arising problem, becoming a major constituent to antibiotic resistance. Moreover, self-medication heightens the possibility of developing...
09 April 2024 6,644 1 View
Antibiotic resistance is a pressing global and local health concern. Outpatient settings play a crucial role in antibiotic consumption and the emergence of resistant bacteria due to the frequent...
09 April 2024 6,004 0 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar
29 July 2024 3,084 8 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View