01 January 1970 1 4K Report

We are trying to do whole-cell patch from hippocampal slices. We use Multiclamp 700B and pClamp 10 for recording. It is hard to break the cell after getting a good seal, even with bigger pressure. When we tried to use Zap, the cell dropped or dead. It looks like the electrode tip was clogged. Does anyone know what might be the problem?

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