Hands-free isolation of CSF from mouse brain?

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Does anyone knows about any protandrous fish inhabiting the Mediterranean Sea, smaller than 20 cm (adult) and that change sex relatively quickly?

Other than Sparids

27 June 2024 1,809 0 View

Very urgent. Where we can get some of these plant species (details see description box) in Nigeria?

1. Vachellia drepanolobium 2. Vachellia horrida 3. Vachellia karroo 4. Vachellia nilotica 5. Vachellia oerfota 6. Senegalia polyacantha ? [email protected] +2348061166247 Thank you.

05 June 2024 4,858 1 View

How can I change the coordinate system in Abaqus using Python?

Hello, I'm writing Python scripts for Abaqus and I'm facing a problem. I need to change the coordinate system in a .odb file before extracting data but I'm stuck. I can create my coordinate system...

30 May 2024 7,431 3 View

Can you explain the difference observed between my PFGE and WGS results for E. coli comparison?

Recently i did PFGE and WGS comparison with the same set of E coli strains. In my mind, if PFGE give two different pulsotypes, it is sure that strains are not the same. But when i did WGS, i...

12 May 2024 8,107 2 View

Is there a commercial antibody against insect resilin?

Dear community, I want to perform an analysis of the risilin localization within the legs of myriapods and would like to visualize the localization of the resilin components in different...

07 May 2024 9,160 3 View

How do I specify the argument "time" in the function "create.matrix" (R package fossil)?

I would like to use the "create.matrix" function (R package "fossil") to create a matrix of taxa, localities, time and abundance. In the function, time is specified by the arguments "time.col" (=...

28 April 2024 3,545 2 View

My plasmid DNA is 96ng. How do i calculate the volume of bsa I (20000 U) to use to digest it?

I am trying to do a golden gate cloning but the ligation-digestion seems to be failing

01 April 2024 6,416 2 View

Handling procedure of brain samples for RT-PCR / Western Blot?

Hello, What would be the best procedure right after extracting brain samples from mice, if we plan to analyze the samples later ? Would it be best to just directly flash freeze the brain...

28 February 2024 7,384 1 View

What should I study to get a good introduction to combinatorial game theory?

Right now I'm working on a senior project in combinatorial game theory - I've read Knuth's book "Surreal Numbers" and I've started reading "Winning Ways for your Mathematical Plays" by Conway,...

20 February 2024 1,254 1 View

Writing a United Nation position paper for poverty and inequality Bangladesh?

What is the Current situation with poverty and inequality in Bangladesh? What are the potential sources/causes of poverty and inequality in Bangladesh? What are the consequences poverty and...

17 February 2024 4,166 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

Louis-Charles Béland

You certainly can't as this would involve surgery and anesthesia.

Sorry

Benjamin Jurek

Well, surgery is fine beforehand. I just need to collect it while the snimal is able to move and behave freely

Ícaro Raony

A novel chronic dural port platform for continuous collection of cerebrospinal fluid and intrathecal drug delivery in free-moving mice (Article A novel chronic dural port platform for continuous collectio...

)

I hope this work can help you!

Uh, thank you, that sounds interesting!