Benjamin Jurek

7 Questions 15 Answers 0 Followers

Questions related from Benjamin Jurek

Hands-free isolation of CSF from mouse brain?

I need to get cerebrospinal fluid from freely moving/behaving mice without stressing them. How would you do that?

19 October 2023 7,671 4 View

RdTomato INSIDE extracellular vesicles?

Might be a stupid question but I can’t find papers about it: can fluorophores (rdTomato, EGFP etc) be taken up by EVs and transported, or do they only get attached to the surface? All I could find...

09 October 2023 4,565 1 View

What video program do you use to record animal behavior?

Hi, i used to record mouse behavior with AnyMaze, but if I want to analyze the videos with DeepLabCut or other software I just need the mp4 file, which AnyMaze isn't giving me. Instead I have to...

24 January 2023 7,202 4 View

How to track mouse/object or mouse/zone interactions with DeepLabCut or SLEAP?

Hi, I am using AnyMaze to track mice in different zones of their homecage and their interaction with object, however, AnyMazes tracking is not really adequate. It often confuses front and back of...

23 November 2022 7,336 4 View

How to stain spheroids? Do you recommend tissue clearing?

I'm looking for a quantifiable way to visualize cell-cell contacts in a 3D spheroid. So far, my stainings have somehow worked, but not to my fullest satisfaction (blurry, uneven staining of core...

27 May 2021 2,779 2 View

What is a good signal peptide to add to your N-terminal GFP-labeled GOI?

I have a cell line that stably expresses an EGFP-labeled GPCR. I know that most GPCRs don‘t require a signal peptide, but my protein levels are rather low, and I would like to boost translation...

19 March 2021 2,844 6 View

Which Affiliation to state? Current or where the work has been done?

When writing a paper, should the current Affiliation of authors be stated, or the Affiliation where the author has produced the data? Is there a general guideline?

01 January 1970 9,452 12 View