Dear all,

I am planning to do histology on frozen sections of hard tissues (mouse bones). I treated the samples as following: 4% PFA for 4h, then sucrose gradients (10%, 20%, 30% with 24h each), embed in SCEM, and section it with Kawamoto´s film. Now I would like to try H&E, and Acian blue - nuclear fast red stainings on my frozen sections, but I am not experienced with frozen sections before, so I would be very happy if you could share me a protocol or some tips. Also, I wonder if it is better to place the sections directly on the slide (and do you use coated or non-coated slide?), or transfer them on Kawamoto´s film prior to placing on the glass slide.

Thank you very much in advance, and I am looking forward to learning from you.

Cheers,

Mai