What kind of simulation i can use to simulate design contain LED and CMOS and nanoparticle sheet?

what kind of simulation i can use to simulate design contain LED and CMOS and nanoparticle sheet?

10 August 2020 3,471 1 View

How can I dissolve thymoquinone loaded on chitozan nanoparticles in culture media with out change ph of the media?

I have a problem in dissolving thymoquinone loaded on chitozan nanoparticles in culture media to make further dilutions without changing ph of the media, can i use DSMO for and how can I preserve...

02 August 2020 931 3 View

What is the best concentration can be used for immunocytochemistry on tissue culture?

I will test cytotoxicity of a drug on cancer and normal cell lines then iam planning to make immunocytochemistry, what are the concentrations can I use for immuno papers differ in this point

19 July 2020 2,296 3 View

What's the best checklist for quality assessment of retrospective studies? Can I use PRISMA checklist for that or not?

I'm working on systematic review (epidemiology of disease) based on retrospective studies( most papers ) and surveys, I need to apply checklist to assess quality of papers

03 May 2019 6,322 5 View

How can i solve this problem in impedance curve ?

13 August 2016 3,241 6 View

How to create the initial conditions for integro differential equation ?

I have a question for how create the initial conditions for differential equation or integro- differential equation ? Is there any specific conditions to be identified?

26 July 2016 1,635 2 View

Where can I get a full soft copy from Thinking Dispositions Questionnaire (TDQ)?

Thinking Dispositions Questionnaire (TDQ) developed by Stanovich and West (1997, 1998, 2003)

17 March 2016 1,640 3 View

Looking for (Remote) Research Assistant in DL/ML

We are an active Arabic Natural Language processing (NLP) and AI research group doing research in Deep learning, machine learning and social network analysis for Arabic NLP. We are looking for an...

01 January 1970 7,840 3 View

What are the desired characteristics of electrospun nanofibres, for oral drug delivery?

Purpose of electrospun polymeric fibres are to encapsulate a drug for oral drug delivery; what does a 'good' fibre look like in terms of morphology, chemical, physical, and mechanical...

01 January 1970 9,116 3 View

As an early career researcher how to be productive and increase your citation??

The current academic climate is highly competitive. Both big scientists and peer-reviewed Journals search for prolific researchers to collaborate with or to publish their work. How to overcome...

01 January 1970 9,764 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View