I have done partial 16s fir these strains with no issues, but it seems to be a common problem in my lab that none of the erics are working.
Dear Rhiannon
Change the technique used for DNA isolation.Probably u may use some kit .
need to know the AA residue and what has changed from the original protein seq.
11 December 2014 1,917 12 View
What program is user friendly, good for cleaning up DNA sequences and aligning the sequences so i am able to do a Standard Nucleotide BLAST? I'm using a mac and am tossing up either Mega5 or...
31 December 2013 4,901 25 View
There is plenty of decent genetic evidence that the mitochondria has a bacterial origin, the structure of the chromosome, the arrangement and regulation of genes, double membrane etc. But as far...
31 December 2013 6,126 6 View
How does the reverse transcriptase know only to target the viral RNA and not the host cells RNA?
09 October 2013 5,528 5 View
Cells can be damaged or killed by excess glutamate, what is the mechanism behind this process?
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In people with Parkinsons disease, dopamine is often looked at as a therapeutic agent, how does dopamine help and would this therapy work?
08 September 2013 2,011 5 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View