I have done partial 16s fir these strains with no issues, but it seems to be a common problem in my lab that none of the erics are working.
Dear Rhiannon
Change the technique used for DNA isolation.Probably u may use some kit .
need to know the AA residue and what has changed from the original protein seq.
11 December 2014 1,953 12 View
What program is user friendly, good for cleaning up DNA sequences and aligning the sequences so i am able to do a Standard Nucleotide BLAST? I'm using a mac and am tossing up either Mega5 or...
31 December 2013 4,949 25 View
There is plenty of decent genetic evidence that the mitochondria has a bacterial origin, the structure of the chromosome, the arrangement and regulation of genes, double membrane etc. But as far...
31 December 2013 6,152 6 View
How does the reverse transcriptase know only to target the viral RNA and not the host cells RNA?
09 October 2013 5,574 5 View
Cells can be damaged or killed by excess glutamate, what is the mechanism behind this process?
08 September 2013 6,393 5 View
In people with Parkinsons disease, dopamine is often looked at as a therapeutic agent, how does dopamine help and would this therapy work?
08 September 2013 2,051 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View