Why do my NIH 3T3 cells die after single cell sorting?

I have been sorting NIH 3T3 based on GFP positivity for quite a long time and this has worked fine when all the GFP positive cells are sorted to the same well (of a 24 well or 12 well plate). The...

05 June 2014 2,294 10 View

Use of ELISA for protein-protein interaction detection

I'm trying to optimize an ELISA-based technique where I coat with a protein, block, add a second protein and then detect for the second protein. Do you know any effective protocol? Also, how...

02 March 2013 7,005 7 View

ELISA coating buffer for acid protein

31 December 2012 7,179 10 View

How fast should an ELISA test be?

31 December 2012 447 9 View

Can I re-incubate ELISA plates with primary Ab?

I'm running some ELISA experiment plates coated with an extremely expensive protein. However, at the end of the experiment there was no signal. The last step of this experiment was the incubation...

31 December 2012 7,737 7 View

Solubilization of proteins from inclusion bodies: 6M Guanidine or 8M Urea?

09 October 2012 9,286 16 View

Will an His tag decrease solubility of a human protein expressed in E. coli?

I have already tried with GST but got some inclusion bodies issue and then I thought His tag would be more suitable. However, I have been reading about it and it seems that in many cases it can...

09 October 2012 8,524 8 View

How can I get my GST fusion protein to bind Sepharose 4 Fast beads in the presence of 0,1% Sarkosyl?

09 October 2012 3,663 13 View

Protein precipitates after GST purification, how to avoid this?

09 October 2012 4,326 11 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View