Methods of Thawing Frozen Mammalian Cells?

I am looking for different methods of mammalian stem cells freezing and thawing. Both in industrial and laboratory scale. Would greatly appreciate your input.

01 February 2019 6,339 11 View

Can someone suggest a material with high range of stiffness?

I am looking for a material to be used as substrate in cell culture study with high stiffness. varying from 5-10 MPa.

06 July 2013 3,091 8 View

Xrd vs DSC

To study crystallinity of materials, which method is better? When is it more accurate to use DSC or XrD?

04 May 2013 4,046 9 View

Clay activation

Which method is better to have an acid activated clay? Heating the clay in a solution of sulfuric acid or using a solution of sulfuric acid/nitric acid?

01 February 2013 3,375 5 View

How can I disperse MMT nanoparticles within carrageenan- or acrylamide-based hydrogel?

Is there any surfactant needed? My MMT nanoparticles have hydrophilic behaviour.

31 December 2012 184 1 View

How can I prove that PVP is wrapped over MWCNT nanoparticles?

I am preparing CNT nanocomposite hydrogel. I have used PVP as surfactant. From the literature, I found that PVP is suitable to disperse CNT NPs in water, as PVP will wrap over CNT wall, but could...

31 December 2012 1,694 15 View

Is there any possibility that mRNA sent from nucleus the geta lost or do not attach to ribosome?

How does mRNA find ribosome in cytoplasm?

31 December 2012 2,509 3 View

What is the difference between Stem Cell Differentiation and Proliferation? In which stage do they happened?

I I could not find any clear answer when searching in the web, does any one can help?

31 December 2012 1,189 10 View

Why are we able to understand the universe and its mysteries?

As far as I know, according to Darwin's Theory of Evolution, we need our intelligence and skills to survive. On the other hand, we are also able to understand the most profound concepts of the...

31 December 2012 8,298 12 View

How is it possible to extract the ion absorbed in hydrogels after the process?

I have used hydrogels to remove heavy metals (CU, Ni, Co) from water. Is it possible to separate these ions from polymeric network without destroying hydrogel network? for the sake of reusing...

31 December 2012 3,581 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View