I want to calculate the concentration of the bacterial biomass of Lactobacillus fermentum based on the optical density of the samples taken. I want to have a very simple technique to do this. Thank you in advance.
Sincerelymnt,
Hey there Yaya LEYGNIMA Ouattara! Well, diving into the world of optical density and bacterial biomass, my friend Yaya LEYGNIMA Ouattara, it's like peeling an onion—layer by layer.
Here's the deal: Optical density and bacterial biomass concentration are buddies, but it's a bit of a complicated relationship. Optical density is commonly used as a quick and dirty measure of bacterial growth, but the catch is, it's not a perfect one-to-one correlation with biomass.
Now, for a simple technique, you Yaya LEYGNIMA Ouattara might want to consider calibrating your optical density readings with a standard curve. Take samples of known bacterial biomass concentrations, measure their optical density, and create a graph. Then, when you're working with unknown samples, consult the graph to estimate their biomass concentration based on the optical density.
But let me tell you, my friend Yaya LEYGNIMA Ouattara, simplicity is sometimes deceptive in the world of microbiology. Keep that in mind as you Yaya LEYGNIMA Ouattara embark on your bacterial biomass adventure. Anything else on your microbiological agenda?
There is no universal relationship for at least two reasons: (1) different bacteria absorb and scatter light differently depending on their size, shape, and surface composition/features and the characteristics of the light, and (2) there is a non-linear relationship between light absorption/scattering and concentration as the concentration increases. However, for a given species of biomass bacteria in low concentration, the concentration should scale proportional to the light absorption/scattering over a reasonably large range.
Yeas, there is a direct relationship between optical density and bacterial biomass concentration
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