Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View

Is it possible to full-couple the interFoam solver with the chtMultiRegionFoam solver?

We need to calculate multiphase stream of the one substance in the air atmosphere (this is why we using interFoam), but also we need to get heat transfer data between the substance, solid obstacle...

11 January 2021 872 2 View

Does anyone have experience with Land use/land cover data extraction from Second military survey maps?

I am trying to create polygon shapefile with land use from Second military survey using Arcmap. However, I really have hard time with reading the map, more correctly with boundaries between...

07 November 2020 6,018 3 View

How do I remove Bacillus sp. spores from Kraft Paper machine?

In the pulp and paper company that I work in, samples from paper, water and pulp from the paper machine gave information about existing spores of Presumptive Bacillus cereus. Our paper is used...

03 November 2020 1,726 10 View

What volume of a cell lysis buffer does one use for cells?

I have certain cells that are contaminant cells in a suspension of cells. I found a buffer that selectively removes them, but a lot of protocols don't specify what volume one should use for that...

03 November 2020 6,330 3 View

Best protocol to perform Western Blot from mice brain?

I am trying to look at the expression of c-fos nuclear protein in mice brain using WB technique. Can anyone suggest a protocol to extract the proteins? Better harsh homogenate before sonication?...

27 October 2020 5,272 2 View

Checking purity of sperm suspension?

I have sperm cells extracted from epididymis of a mouse. I would like to check and quantify their purity. My first idea is to use an intercalating dye like Hoechst and then see what fraction of...

25 October 2020 8,600 1 View

What do you think now of virtual teaching compared to face-to-face teaching of finished transmission of knowledge?

21 September 2020 4,178 10 View

How to store W/O droplets for over 10 days using paraffin oil with Span 80?

I am currently using a PDMS chip to produce water droplets with a diameter of 200 um in paraffin oil with 3wt%. After a couple of hours, the stationary droplets stored in the chip starts to form...

18 August 2020 2,459 3 View

How do you use factor analysis on categorical variables?

Hi, I'm trying to identify how similar different individuals are in their body traits. Normally, I would use a PCA or DFA, depending on my question. However, I have only categorical variables...

20 July 2020 4,171 1 View

Do drugs that are injected i.t in rats reach the supraspinal system and how long does it take to clear them from spinal cord?

I am injecting intrathecally (between L5-L6) , in rats, cannabinoid analgesics and I was wondering if they can reach the brain and have effects there as well ? Also I need to pretreat with...

07 January 2021 5,397 4 View

How can i properly obturate more than one canal at the same time in one tooth using CLC without interfering with the canal positionand accessory file?

#Rootcanal #Obturation #Endodontics

29 December 2020 5,982 2 View

Pearsons correlation sample size HELP?

I'm conducting a correlational study about "is there a relationship between depression, acceptance and quality of life among spinal cord injury patient?" But I am having an issue determining the...

06 November 2020 9,798 10 View

How to increase zebrafish survival rate after spinal cord injury ?

Hi everyone I'm having trouble with the high mortality rate of my zebrafish after surgery. ( the surgery is done based on this article: Becker, C. G., Lieberoth, B. C., Morellini, F., Feldner,...

13 September 2020 6,781 1 View

How do you embed spinal cord in paraffin and how do you recognize ventral horn?

Hi I am going to slice paraffin embedded spinal cord. I have two questions: 1. How do I put the SC in the paraffin block so that it will be in coronal orientation? It is not trivial as the part...

13 August 2020 7,623 2 View

I want to measure cytokine levels in mouse spinal cord tissue and I can not decide which method is best, a cytokine array or ELISA?

Hi everyone. So I need to measure different cytokines in mouse spinal cord tissue and I can not decide which method is the best one, a cytokine array or ELISA. Does anyone that has experience in...

26 July 2020 1,757 2 View

Which microglial cell line will be useful in studying inflammation in the spinal cord?

Most of the studies use primary cells or stem cells for modelling the spinal cord, but as I never worked with these cells, I would like to start with a cell line. Can anybody suggest a good...

07 July 2020 4,248 2 View

Where does the notion of "Central Nervous System" (including both the brain and the spinal cord) comes from?

I mean, you can easily find out that Galen was the pioneer, correcting Aristotle; and a bunch of other intersting historical things. But WHO exactly decided to establish this partition, WHEN, and...

11 March 2020 5,083 2 View

How much is mouse full body resistivity order?

I need to know the mouse body resistance specially in spinal cord region. Any one has some info about it??

01 February 2020 1,395 1 View

How can we validate intrathecal administration in mouse spinal cord?

Beside color marker injection, is there any pharmacological agent (beside morphine) that induce a particular phenotype (morphine Straub tail) in mice? thanx in advance Roland

14 November 2019 5,968 4 View