Good evening all. I would like to ask there are specific pcr conditions when cleaning up a pcr product before using the sanger sequencer?

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Which Software is used for drawing Illustrations / Diagrams such as those attached?

I would prefer suggestions of both Open source and Commercial software packages

13 December 2023 1,017 11 View

Has South Africa started using or used natural gas before, to generate electricity into the national grid?

The national utility Eskom has been converting (since 2017) its OCGT power stations to also run on natural gas after the discovery of gas fields. From the historical data given by BP's Statistical...

12 August 2022 8,376 5 View

What the diferencence between municipal and public international law?

the definations and diferences between the 2 laws

24 February 2020 2,146 3 View

What is the future of regional languages as mediums of instruction in Africa south of the Sahara?

What is the future of regional languages as mediums of instruction in Africa

07 January 2020 7,090 1 View

How can i come up with a sediment yield model using spatial system dynamics modeling?

Its a research am conducting that requires that I come up with such a model and the model must include climate change and land use/ land cover change Study area is Tokwe sub catchment in Zimbabwe

02 January 2020 9,494 1 View

What is the policy position of SSA governments (eg Zimbabwe) with regards to Integrated Catchment management?

1. Current literature on this issue of Integrated catchment management

28 January 2016 622 1 View

What is the best protocol for Pythium irregulare zoospore production?

18 November 2015 220 3 View

Do synergistic effects mainfest themselves in the analysis of coal-biomass blends?

Synergistic Effects in Gasification of Coal/Biomass Blends: ... I suspect synergistic effects can manifest themselves in the ash and volatile content analysis following the ASTM standard...

01 January 1970 8,134 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is there any machine to do real time pcr?

I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering

05 August 2024 1,660 0 View

Dirty and clean?

Hi everyone I need a file with a dirty and clean potato image

04 August 2024 7,199 4 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How to clean the CAD detector?

The module of the CAD detector is corona Veo. It has been idle for a long time. And it was not well maintained before idle. I have replaced the gas filters and flushed the system with a 50/50...

01 August 2024 7,805 0 View

Which filtration method to go for run off water from dirty solar panels to be used again?

We are working on a robot that cleans solar panels using fresh water supply and a rotating brush. We are trying to conserve as much water at possible by recycling the dirty water that is collected...

28 July 2024 5,778 2 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View