i want to test the susceptibility of Salmonella species to some plant extract want to know if there is a standard for choosing concentration to be tested.
You should do a review on the plant extracts, you have:
If these extracts have been tested against Salmonella species, you could refer to the tested concentrations as basis to check the best concentration of your extracts; while keeping in mind that the evaluation method and the experimental conditions may influence this concentration
If your extracts are not yet tested against Salmonella species, then you should do by yourself the screening of extracts, by choising different ranges of concentrations (reffering to similar studies with the same methods to that of which you want to cary out) ; for example you choose 1mg/mL, 4mg/mL and 10 mg/mL, to test the susceptibility of your pathogen. if thereafter, the concenttration 4mg/mL totaly inhibit your pathogen, and not that of 1mg/mL, you could check the best concentration of your extract between 1mg/mL-4mg/mL.
Hallo,
The methanol extracts from the root or leaf of 8 traditional medicinal plants showed various degrees of the inhibition against 15 bacterial strains using the agar dilution method.In this method they have tested inhibitory effects against Salmonella species also ..
May it will help you.
Please go through below article
Article Evaluation of Antimicrobial Activity of the Methanol Extract...
It depends on the concentration of your atnimicrobial active ingredients in the extract. It is good to check extremely low, low, moderate, high and extremely high concentration ranges. This approach will also help you to determine the minimum inhibitory concentration (MIC) of the extract.
For each plant and every indivudual extract the inhibition activity as well as the concentration differes. There are no common standard concentrations. If the plant extract was already reported against your particular culture, take the maximum concentration which sowed higher activity as reference from that you can two lower and two greater concentrations with that you optimize for your extracts.
If there are no literature on your plant extract, you can find some other relative plant of the same genus, and do the same described above.
If not the least option will be you optimize the concentration by your own. like 10, 50, 100, 200, 400, 800, 1600, 3200micogram likewise you can increase the concentrations and optimize the proper concentration for the study.
Dear Temitope
One way is determination of MIC and MBC of extract against the bacteria. another way is disc difusion method on an agar media with different concemtrations of extract to determine the suseptibility range of bacteria for mentioned antimicrobial.
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