01 January 1970 0 6K Report

What is it in your opinion a good book for an introduction on phytopharmas with focus on chemistry and synthesis?

More Valentino Russo's questions See All
Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Strategies for short hairpin RNA interference cassettes?

In your experience, how much does the distance between the U6 promoter and the annealed -short hairpin - oligonucleotides affect the RNAi cassette expression? How to clone a shRNA sequence...

16 January 2021 9,418 2 View

How to run a statistical comparison of same group and 2 conditions in fMRI?

Hi everyone, I have some doubts about which statistical test to apply in order to test the probability of eventA to happen at the same time of non-eventB. Basically I have my fMRI group with...

14 September 2020 1,887 3 View

What is the best way to approach building a custom polypeptide?

I am trying to build a custom poly peptide that is ~50 amino acids in length, and it will ideally have a charge of ~+4 at pH 7. I can get the positively charged attributes from incorporating a...

08 December 2019 3,597 1 View

Olsen method, problem with calibration curve. How to fix?

Dear all, we are trying for the first time to perform the quantification of P with the Olsen method. We had followed the protocol proposed by Guide to laboratory establishment for plant nutrient...

20 September 2019 1,116 4 View

Whole Blood Over Recovering Compared to Blood Plasma?

My Immunoassay is giving higher analyte concentrations for the same sample before plasma separation vs after plasma separation. For instance, I run my assay with whole blood, and I get a reading,...

10 July 2019 7,391 3 View

Is there a valid and reliable scale for measuring the quality of student-teacher relationships from the student perspective?

Is there a valid and reliable scale for measuring the quality of student-teacher relationships from the student perspective, that has been used with primary age children? Is there any research to...

04 June 2019 10,015 1 View

What kind of emerging educational technologies can be utilized within a Science (Physics) learning and teaching environment?

What kind of emerging educational technologies can be utilized within a Science (Physics) learning and teaching environment?

11 October 2017 7,927 4 View

PCR: lower bands. How overcome high Tm?

I am trying to amplify the luminal domain of TRAP alpha subunit (ER), but probably TM is too high. I performed PCR with two different pairs of primers but I get bands below 500 bp (see picture),...

12 July 2017 1,156 10 View

Why my frequency calculation aborted?

Hello, I am trying to running a frequency calculation with gaussian for a dimer that is made by benzene and bromine fluoride at T-shape. After finding a stationary point in the optimization, the...

06 March 2017 6,071 2 View

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How to calculate the RMSD values for a MD simulation using MOE?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

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fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

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