good afternoon. Tell me, RPM of the centrifuge (G) can the suspension of plant cells be precipitated without damaging the cells. thank you?
Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...
16 February 2021 1,664 3 View
Hello, I'm biologist and currently working on MIP. I wanted to know is there any literature that mention the influence of monomer structure (for example size of molecule) on MIP selectivity and...
27 October 2020 1,571 6 View
Hello everyone! I need to etch Si to the depth of 1mm approximately, not affecting the surface of the sample (which is atomically flat and must be kept as it is, so we can't anneal the sample)....
16 August 2020 9,727 1 View
I'm writing PhD thesis and need to compare different drug delivery systems applied to SOD delivery.
22 July 2020 7,210 2 View
What do these substances stain exactly?
20 June 2020 8,653 1 View
You can provide links with calculation and clear examples of the methods used. I would also like to understand how to take into account a specific potential. How to determine the forbidden zone...
09 June 2020 9,728 2 View
Does anyone know any good papers on the impact of the political elite's ideas on institutional transformations in terms of comparative politics? It will be really great if there are both...
27 February 2020 3,665 3 View
I'd like to publish some ideas about taxonomical database. This is possible to do in Biodiversity Data Journal or PeerJ, but I need to pay for an article. Does anybody know similar journals...
23 February 2020 6,876 3 View
Is "green energy" really "green"? Is Green Energy Really Eco-Friendly? Isn't solar panel manufacturing too dirty? Is it not too damaging to put wind turbines in farmland? Don't we pay too much...
24 January 2020 4,067 14 View
Dear All, I am about to start purification of many variants of not tagged recombinant protein expressed in E.coli. I need it to be at least 70% pure. Column purification would be very time...
09 January 2020 7,116 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...
24 February 2021 5,183 1 View
Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...
24 February 2021 2,006 2 View
I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...
23 February 2021 2,390 3 View
Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...
19 February 2021 9,742 2 View
Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...
17 February 2021 1,709 3 View
I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...
15 February 2021 3,746 4 View
I'm currently experiencing problems during acid hydrolysis for total amino acid analysis as my first samples contain high amounts of sugar (I get too low results back). I therefore had the idea to...
14 February 2021 6,049 3 View
Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...
10 February 2021 1,797 5 View