good afternoon. Tell me, RPM of the centrifuge (G) can the suspension of plant cells be precipitated without damaging the cells. thank you?
I am searching for papers that help me answer this question. Furthermore, I need studies that analyse the saving behaviour of polically polarized persons (far left and far right). e.g. do far...
26 July 2024 7,780 0 View
The following are given: Thermal Conductivity of Barrel Material of 37.67 W/(m·K), Barrel Length of 290mm, Barrel Inner Diameter of 16mm, Barrel Wall Thickness of 9.5mm, Barrel Outer Diameter of...
22 May 2024 4,296 1 View
21 May 2024 6,410 1 View
Folks, I need an answer for the methods available to test the High-Temperature & Ultra High-Temperature Composite (Materials), Especially in Aerospace Applications.
14 April 2024 8,681 1 View
Dear Professors/Researchers, Can you please suggest, the latest Additive Manufacturing & Casting Techniques used for Aerospace Applications?
14 April 2024 3,713 1 View
Which is the best coating/Surface treatment/Processing technique available to minimize Nozzle erosion in Hypersonic vehicles?
14 April 2024 8,694 2 View
Are there regulatory requirements for the design and organization of rooms for clinical NGS laboratory?
06 February 2024 1,208 1 View
Do you use any Smartphone App for AR in Neurosurgery?
24 January 2024 5,746 4 View
I am trying to model evaporation of a droplet on a substrate in Comsol. I've managed to reproduce the methodology of the tutorial model "Droplet Evaporation on Solid Substrate" and it works fine....
13 December 2023 8,914 0 View
Is it possible to complain on scientific journal? For example, if the paper was initially accepted by the editor (after passing 2 rounds or review) and later rejected by the same editor for...
11 November 2023 6,674 2 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...
23 July 2024 4,074 2 View
We have no access of pressure plate apparatus
22 July 2024 3,488 2 View
Hi All: I need to precipitate extracellular protein from supernatant. A bit confused with ammonium sulfate calculation. Should I be adding: 1. 65g of ammonium sulfate in 100 ml of...
09 July 2024 5,508 0 View
I synthesize silver nanoparticles ,and reserve it in water. Then, when I sonicate it some minutes or longer ,it couldn't be dispersed, most of them aggregate .Otherwise ,when I sonicate several...
09 July 2024 7,335 0 View
Hello, I recently started working in a soil testing lab and am in charge of operating a new piece of equipment in the lab. We are using 4g soil to 40ml extraction reagent, shaking the mixture,...
26 June 2024 1,265 3 View
I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...
19 June 2024 2,590 0 View
Dear colleagues, does anyone know of a suitable alternative for Centrifuge software (calculating settling time for grain size separation by centrifugation), preferably one running on Windows 10 or...
17 June 2024 7,085 0 View