Hi Research Gate People,
I've been tasked with establishing a Golgi Staining Protocol and I've never done Golgi Staining.
Is there a commercially available kit for Golgi Staining? If so, who makes it?
Thank you.
Dear David Hubbard many thanks for sharing this very interesting technical question with the RG community. First of all, please note that I'm absolutely no specialist in this field as we work in the field of synthetic inorganic chemistry. However, I can recommend to you the following detailed protocol which will certainly be helpful for you:
Golgi-Cox Staining Step by Step
Article Golgi-Cox Staining Step by Step
Fortunately this article has been posted by the authors as public full text on RG. Thus you can freely download it as pdf filed and get started right away.
I hope this helps. Good luck with your work and best wishes, Frank Edelmann
Thank you Frank T. Edelmann The article is a good explanation of the process. It answered a question I had about tissue preparation, letting me know not to perfuse with PFA.
I also found a video from FD Neurotechnologies about using their kit which looks simple. They froze sections to -80 and used a cryostat to section the tissue. It wasn't mentioned if brains were in 30% sucrose prior to the freezing process. Does can anyone comment on if sucrose is used as a cryoprotectant prior to freezing in this procedure?
Link to video
https://youtu.be/VrfJO1xzsCw
I also found an article by Zhong et al. 2019 Frontiers of Neuroanatomy that details a Golgi-Cox procedure.
Hey David,
I haven't done the technique yet but as far as I have read for FD kit:
PFA and sucrose are interfering with the technique just like perfusion
for other methods:
4% PFA 24-48h > 15% sucrose 6-24h > 30% sucrose overnight-48 h
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