Hi, I am trying to analyze Golgi organization in neurons. I’ll appreciate if you could let me the best way to analyze the morphology/changes in Golgi in neurons. We are trying to analyze Golgi phenotypes (fragmented, diffused or condensed) in different groups of neurons. We have stained cultured neurons with GM130 and TGN38. Are there any tools in Image-J by which we can define the different pattern of shape and distribution pattern of Golgi inside cultured neurons? Thank you.
Saroj: If you are familiar with MATLAB, there is an add in package Within Image Analysis called as " Granulometry of Snowmetry". If you can able to do some twirks and turns on few lines of codes, you can able to get your answer. I dont know for sure but CellProfiler is pretty powerful but i guess it needs MATLAB in the background as well. Try that one as well if you are unfamiliar with programming.
Best,
Surya
Surya Prakash Rai Thank you so much. I not familiar with MATLAB. Finally, I used image-J and measured circularity and roundness as morphological parameters for fragmented and nonfragmented Golgi.
Sincerely,
Saroj
- Badges
- Science method
- Similar topics
- Biological Science
- Histology
- Staining