I would like to compare the polystyrene plates we use for ELISA assays to glass. Does anyone have experience either passively coating or covalently linking a capture antibody to a glass surface? I have found methods for covalent linkages, but would like something simpler.
Jeremy
Poly lysine coated glass slides have been coated with antibodies for microarray.
Thank you, Syed. I've come across this strategy, and it seems like a covalent linkage made to the capture AB. I'm hoping to keep the glass as glass. I suppose it's time to scientist-up and try bare glass to see what happens.
Hi Jeremy, we used to treat glass beads with glutaraldehyde. We then proceeded as for plate-based immuno-assays.This method worked well.
See A Polson paper for method. Immunol Invest. 1985 Jun;14(3):223-31
Edmund,
Thank you. The paper title/abstract look interesting, but I don't have institutional access to this publisher. Do you have a copy of the pdf you could send?
Hi Will check if there is a copy in the university library when I go back next week. I will also check with some of the people who studied under Dr. Polson.
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