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Questions related from Jeremy Pronchik
Using a fluorophore rather than a chromophore for ELISA detection seems like an obvious solution to increasing ELISA sensitivity, but I'm struggling to find work done in this area. Can anyone...
08 August 2019 8,715 6 View
free as in unreacted sulfydryls, not free as in speech, nor free as in beer :) I am working with an 80 kDa plasma protein with 5 cysteines, 2 of which are in a disulfide bridge. There are a lot...
12 December 2017 4,735 15 View
Generally we want dyes whose spectra or brightnesses are modulated by their interaction with a species of interest. I need the opposite of that. I am developing a 96 well microplate fluorescence...
11 November 2017 404 0 View
I am investigating whether lipids are bound to my plasma protein of interest and interfering with its quantitation by ELISA. I would like to pretreat samples with something capable of removing...
05 May 2017 7,420 10 View
I do ELISA's, but don't have a long history of interpreting them. I have non-standard data that could use some experienced eyes.Attached are plots of a 2x dilution curve detected by sandwich...
03 March 2017 9,107 4 View
I would like to compare the polystyrene plates we use for ELISA assays to glass. Does anyone have experience either passively coating or covalently linking a capture antibody to a glass surface? I...
02 February 2016 5,420 6 View
