Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Do we have any empirical model to model the combined effect of carbonation and chloride attack in concrete concerning climate change?

Do we have any empirical model to model the combined effect of carbonation and chloride attack in concrete concerning time-dependent temperature, relative humidity and concentration of carbon dioxide?

08 February 2021 4,277 1 View

Does AMBER or CHARMM force field parameters have coarsed grained side chain parameters?

I need to coarse grain the side chain with a single atom. However, what force field parameters do I have to use. Does AMBER or CHARMM have any parameters and partial charges for coarse-grained...

31 January 2021 7,091 3 View

Where can I read the force field parameters of amino acids and protein ?

I am a beginner in the field of molecular dynamics and biochemistry. I need the parameters such as van der Waal radii, partial charge of each amino acid. How do I read directly the parameters from...

20 January 2021 3,541 3 View

Can anyone clarify what is the difference between 'R' software statistics and SPSS Statistics?

I recently learned few things about 'R' programming language. It is open source / free platform in many aspects. R helps to descriptive statistics, inferential statistics, multivariate...

17 January 2021 1,089 20 View

Does anyone know what is the minimum variables required for standard deviation? and why large datasets is considered as reliable data?

For Descriptive statistics, we usually find out mean, median and mode as well as standard errors and standard deviation. Almost in research articles, I can see mean with standard deviation only....

10 January 2021 4,913 3 View

For mineral / compound characterization studies like FTIR, SEM, XRD, how many replicates should be taken for data analysis?

If we do some spectroscopy technique for only one sample, is it enough for data analysis or we have to do multiple samples from each treatment for getting authenticate results to publish in...

09 January 2021 5,896 2 View

Is there a way to identify the clay mineral based on the Si:Al ratio that has been obtained through EDAX analysis?

I have come through a few literature,were the presence of particular clay mineral has been validated through the Si:Al ratio obtained from EDAX analysis. But, I would like to understand the basic...

08 January 2021 5,982 6 View

What is the reference for the partial charges values for peptides shown in avagadro?

I built short peptides in Avagadro, using Build--->insert--->peptide. After constructing the short peptides of 4 amino acids(tetrapeptide). Under the drop-down, View--->Atomic...

27 December 2020 778 3 View

What are the application of graph theory to fluid mechanics?

What are the application of graph theory to fluid mechanics? Kind help if any papers kind help Any relation to 1) distance in graphs 2) graph coloring 3) graph Decomposition Or any other fields...

08 November 2020 9,974 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View