getting problem in synthesis of Caumarine chalcones as not getting single spot TLC, can anybody share synthetic protocol for same?
Try to use "Green Reagents" and use less protecting groups etc.minimize number of steps, try to use Flow or Click Chemistry
See attached files
Thank you sir...but we dont have flash chromatography at our college, so any alternative way to get chalcones easily
Have you tried crystallization?
For Flash chromatography see attachment.
do you have Prep TLC for purification of smaller quantities?
Possibly you receive halkon in the alkaline environment. Coumarines in the alkaline environment are unstable and can form salts of oxycinnamon acids. Replace alkali with secondary amines and use an azeotropic removal of water.
Dear Sir, ( V. S. Talismanov ) can u tel me hw to set up this reaction, any paper or method?
I recommend Jürgen's answer
Best Regards
It Seems that the major role is on pre-biotics and its supplement. But as far as the gut health is concerned there is major role of Pro-biotics also. So, i would like to know the expert opinions...
30 January 2024 6,682 4 View
why Lack of Fit P-values show star *
01 January 2024 330 3 View
I am working on ABAQUS to simulate the behavior of a reinforced concrete deep beam. I am facing the issue of the Dilation Angle in that. Can anyone please guide me to solve this issue?
03 May 2023 8,009 0 View
I am working on simulation of savonius hydrokinetic turbine in cfx. I have set inflation layer on turbine as shown in photos. It is observed in the previous investigation that, the non-dimensional...
23 April 2023 2,983 3 View
As there is section, Letter to Editor, does it carries equal weightage as the regular research paper.
03 April 2023 6,580 1 View
I am taking half quantity of oil and half quantity of methanol, than vertex for 10 min to mix it. Than the upper methanolic portion collecting in other tube or vessel and keeping for methanol...
19 March 2023 5,413 0 View
I want to know about the C4 pathway of Plants and the usefulness of C4 plants in the present scenario where consistent Geo-climatic changes are occurring and role of C4 plants in assuring food...
05 March 2023 399 0 View
I am trying to develop a standard curve for miRNA using qPCR. I keep getting Ct vs log(mass) slopes around -5, which is much lower than -3.3 (100% PCR efficiency). I am looking for advice on how...
22 February 2023 4,041 3 View
Does the Shelf Life is different from Expiry for Drugs as well as Food? If it is different then what is the exact difference?
01 February 2023 6,901 1 View
It continuously mailing for paper etc.
08 January 2023 6,584 2 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
How can I measure the thickness of thin film by Uv-Vis?
06 August 2024 7,810 3 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View
or we can collaborate
01 August 2024 6,705 2 View
I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?
31 July 2024 8,415 1 View
Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...
30 July 2024 435 3 View
I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...
29 July 2024 8,034 4 View