Geological map Sri Lanka?

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How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View

How to improve NHBE adhesion after subculture?

I'm trying to subculture NHBE cells from Lonza, however, the cells seem to not adhere to the new flasks after detachment. I've followed Lonza detachment protocol (2 mL/25 cm2 of Trypsin 0.05% and...

10 February 2021 196 1 View

How to perform Potential Surface Energy Exploration (PES) calculations?

I would like to receive help, as I am trying to make an analysis of the ethanol PES, but the calculations are always giving error. I use the conda-forge version of xtb (* xtb version 6.3.2...

04 January 2021 602 2 View

How to determine Forster radius of a FRET pair?

Dear all, I'm searching for methods to calculate Forster Radii of FRET pairs, ideally for water-soluble and membrane dyes. Although I am relatively familiar with working with microscopy FRET, I am...

07 December 2020 3,051 3 View

Can an algorithm come into control and possession of data without the knowledge of or input from a human programmer?

Keeping in mind the adage on machine learning: data in, data out. I am curious whether there are neural network algorithms that could allow an algorithm to control and possess data on its own...

30 November 2020 860 11 View

What is the relationship between induction machine stator reactance variation and slip?

It is possible to notice, at some three phase induction machine datasheets, that exists a relationship between stator leakeage reactance and slip, that is, the stator leakage reactance increases...

23 November 2020 2,949 3 View

Bloodworms (Chironomus sp) mass production protocol?

Anyone knows an effective protocol to mass produce bloodworms larvae at lab?

26 October 2020 6,998 2 View

Double digestion with restriction enzymes not working. Any suggestions?

Hello there, I'm having some issues with digesting a PCR product and using it for ligation with the vector pET-28a. My PCR product is about ~1200 bp, I purify it and get about 30 ng/uL. Then I...

21 October 2020 2,736 3 View

Is there variability in dorsal glow spots in Lamprohiza mulsantii also in thoracic segments ?

I decided to edit this question to show the right/ correct things and erase those clearly wrong in order to avoid confusion....

03 October 2020 5,889 3 View

Can you have a (very) local weather forecast model with simple weather stations?

Dear metereologists, sorry if this question is too basic, I'm just curious about it So, I`ve seen some personal stations that capture temperature, humidity, wind speed and pluviometry...

27 September 2020 178 4 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

Is it possible to obtain a negative cronbach alpha?

Good evening everyone. If a questionnaire has 4 factors measuring different constructs as for example CAMI questionnaire measuring attitudes toward the mentally ill that has four factors:...

18 February 2021 3,223 3 View

What are the available keys and descriptions for the identification of lice and fleas in domesticated animals?

I am an undergraduate student at the University of Peradeniya Sri Lanka, currently working on ecto-parasites in domesticated animals. I found difficulties in the identification due to a lack of a...

15 February 2021 277 1 View

What are the limits of determining metastability in the brain?

What are temporal limits in determining brain metastability? In other words, how long does the minimum length of the scan segment using fMRI have to be for the result to be of any value? I'm...

07 February 2021 2,380 2 View

Are the hyperspectral image datasets which are publicly available at ehu.eus website atmospherically and geometrically correct?

I've come across a couple of publicly available hyperspectral image datasets from the website http://www.ehu.eus/ccwintco/index.php/Hyperspectral_Remote_Sensing_Scenes Now, my concern is...

05 February 2021 3,550 1 View

I'm trying to do pixel-wise correlation comparison of two datasets? Any available procedure or codes to perform this?

I'm trying to do pixel-wise correlation comparison of two datasets? Any standard procedure or codes to perform this? Like Correlation Coefficient map and RMSE map of a particular region.

31 January 2021 2,959 8 View

Can I use video interview excerpts from others in public domain (YouTube, Vimeo, etc) for my empirical analysis?

Dear community, I am currently writing my master's dissertation. As a part of the method, I have to include views (interview) of experts for the empirical analysis. The problem is I have not...