Generating GAG affinity matrix for protein purification.

25 June 2013 1 10K Report

Does anyone have experience attaching glycosaminoglycans (CS, KS, HA, HS) to GE Healthcare Epoxy-activated Sepharose 6B beads? Thanks.

Bernd Gesslbauer

I would recommend biotinylation of GAG chains and coupling to Streptavidin. In our hands, that worked better than Epoxy-activated Sepharose. You may have more structural flexibility of the GAG chains using a linker like Biotin. This may be very important for the interaction of the GAGs with proteins...

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