in the HELP of the Genpop online of how you can input your data
genepop.curtin.edu. /help_input.html
they input the allele size of the locus as in the attached file
I ask about the homozygous of allele size 90
it was written as 090090
this means that it has only one band of 90 bp ?
but another one was written as 000230
this means that it has one band at 230 bp
why it wasn't written as 230230?
is this not considered homozygous
Dear Dragos,
i used GenALEx to prepare my input file , but i want to know when there is one band in the gel the allele will be written in the column of locus (the same size twice or one zero and the other the size of the band)
thanks alot
Hi Ergy,
I belive that should be only an example of input file with microsatellite alleles. However when you see clearly and unequivocally one band, you can considere as homozigosous locus (230230). But if in your gel you have one band not clear or with other frangments in your sample, then it could be an missing allele (000230). Anyhow, in this case I suggest you to remake your PCR, modifing the concentration of DNA template and other PCR reagents.
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