Hi everyone!
I've been study the expression of 5 microRNAs using TaqMan assays in several cell lines (microRNAs were selected through literature review) and I obtained statistically significant results in spite of the results were not the expected.
Is it possible now to perform gene ontology studies and construct networks with the cytoscape to better understand the role of these microRNAs in my samples without a previously global microRNA expression analysis?
Hello Anna. Yes, it is possible.
You can use Genemania to perform a iteration network between target genes. If you have not yet found the target genes for microRNAs, use the MiRTargetLink Human tool to find them.
Hi @Wanderson Gonçalves
Thank you so much for your answer. I was afraid that it couldn't be possible since all the papers that I have been reading concerning cytoscape and gene ontology, they first perform a global microRNA expression analysis and only then they confirm the significant results using the TaqMan Assays.
You can use a list of predicted targets from any miR database, better selecting genes with high predictive score. As an alternative, you can search for a publicly available dataset similar to your work and analyze their data according to your aim.
Iman Hassan Ibrahim thank you so much for your answer.
If anyone has a different opinion please let me know!
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