Hi,

I am studyin gene expression of BDNF and VEGF in mice brain through qPCR. The brain sections are embedded in OCT and are very fragile, hence hard to seperate hippocampus. I found in the literature that the gene expression profile is mostly performed on hippocampal mRNA, but I guess those studies involve learning process. However, in my case, its not related to learning but the impact of disease on brain (more specifically if its causing stress in brain or not).

Now, I want to know what information am i loossing if i use whole brain instead of hippocampus to study those two genes? will it be worth if i find a difference in whole brain? or should I tr y something to work specifically on hippocampal!

I am not an expert in neurology so any suggestion will be appreciated. thanks