Matti Ullah

4 Questions 9 Answers 0 Followers

Questions related from Matti Ullah

Shift of population to high SSC low FSC in T-cell culture, am i missing something in media?

hi. i have a peculiar problem while culturing PBMC from human as well as mice splenocytes. for the picture part, I isolated T-cells using Ficoll-paque gradient (sigma), and then depleted...

22 July 2020 8,560 3 View

Gene expression: Whole brain vs hippocampus?

Hi, I am studyin gene expression of BDNF and VEGF in mice brain through qPCR. The brain sections are embedded in OCT and are very fragile, hence hard to seperate hippocampus. I found in the...

29 April 2020 5,035 0 View

Presence of protein in a cell line that is used as a negative control for that specific protein?

I have quite an absurd issue. for the information: 1) I have a cell line (K), that i have seen in literature being used as a negative control for my protein (P). However, for me the cell line...

31 May 2019 3,369 3 View

Antibody washes away from cells after centrifugation, FACS?

Hi, I am experiencing an absurd problem while doing FACS. While staining OVCAR-3 cells with CD90 marker (for titration), i found that antibody washes away after centrifugation (which I guess...

15 October 2018 1,601 4 View