yes dna is very stable and even a large amount of degradation will leave dna long enough to pcr amplify and at -20 there will be almost no degradation. It will be fine.

yes dna is very stable and even a large amount of degradation will leave dna long enough to pcr amplify and at -20 there will be almost no degradation. It will be fine.

Paul Rutland Thank you so much!

Hi Chea,

gDNA would be stable at -20 for months. However, avoid freeze thaw and it would be better to store in aliquots if you intend to use for a longer period.

Bhuwan Bhaskar Ah okay. Thank you so much!

Bhuwan Bhaskar : Actually, unless you intend to do long PCR, freeze-thawing will be OK:

Article Characterization of Effect of Repeated Freeze and Thaw Cycle...

Hope it helps!

Thanks, all, for your inputs! :)

Dear Rachel Andrea Chea,

If you isolate your gDNA on Fridays and you use it on the next Mondays, you can store the gDNA without any problems in a normal refrigerator at about + 4°C!

In my eyes this is the better procedure!

You can keep off the first freezing/thawing cycle.

Best regards,

Thomas

Storage of extracted gDNA at -20 is okay as it is not degraded! However, from experience, it is normally best to work with fresh samples for any upstream experiments for instance setting up a PCR. The stored gDNA is sometimes compromised in the quality of the results obtained as compared to working immediately after extraction