Hello Marwan,

I am unsure if I understood the question well, but you could use potato dextrose agar (PDA).The fungal mycelia will grow on the medium and be visible as a network of delicate, branching structures. Here are the steps to perform the PDA assay:

Obtain plant tissue: Collect plant tissue you suspect may be infected with fungal mycelia.

Sterilization: To avoid contamination, sterilize the plant tissue by exposing it to 70% ethanol for 2-3 minutes, then rinse it with sterile distilled water.

Cut and place the tissue on PDA: Cut the sterilized plant tissue into small pieces and place them on a PDA plate. Seal the plate with parafilm to prevent contamination.

Incubation: Incubate the plate at a temperature suitable for the fungus, typically around 25°C, for 3-7 days.

Observation: After incubation, you should be able to observe fungal mycelia growing on the surface of the PDA around the plant tissue.

This simple method can be used to detect fungal mycelia in plant tissue. However, it is essential to note that the growth of the mycelia will depend on the conditions in which they are grown and the specific species of fungus.

I hope this helps. Yours sincerely,

Edgar M Cambaza