How to perform amino acid conservation analysis of Dynein heavy chains??

I have dynein inhibitors, and want to prove that they inhibit the dyneins of Leishmania (my model organism of interest). I would like to perform an amino acid conservation analysis where I can...

10 November 2017 5,864 3 View

In flagellar/ciliary motility what is the exact definition of a principal and reverse bend of beating flagellum/cilium?

This is something that has always got me confused. I come across so many papers but have not been able to find out on what basis does one basically categorise these two bends (principal and...

02 March 2017 3,887 0 View

What is the volume of a single Leishmania cell?

Can anybody suggest any references where the cell volume of Leishmania promastigotes has been measured?

04 May 2016 9,011 3 View

Mechanism of action of Dipyridamole?

I would like to know how effective a phosphodiesterase inhibitor is Dipyridamole? Does it inhibit both cAMP and cGMP specific PDEs? How effective is it? Any references will be helpful!

05 June 2015 7,835 0 View

Does anyone have experience with cloning and over expression of a large protein approx 450-500kDa?

08 September 2014 5,293 3 View

Can anybody suggest me any publications where the concentration of protein (say dynein) needed to perform an ATPase assay has been mentioned?

I would like to perform an ATPase assay on purified dynein, however I am totally clueless on what should be the concentration of the pure protein with which I perform the assay. Any suggestions or...

07 August 2014 5,898 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View