From where can I purchase the phosphodiesterase inhibitor CpdA?

Aakash Mukhopadhyay @Aakash_Mukhopadhyay

05 May 2015 1 565 Report

I came across this trypanosome specific phosphodiesterase inhibitor CpdA in few papers. However I could not find any company which sell the same. Any leads?

Gianni Colotti

The original library is Nycomed Takeda. They should sell the compound.

Badges
Science topic

More Aakash Mukhopadhyay's questions See All

How to perform amino acid conservation analysis of Dynein heavy chains??

I have dynein inhibitors, and want to prove that they inhibit the dyneins of Leishmania (my model organism of interest). I would like to perform an amino acid conservation analysis where I can...

10 November 2017 5,967 3 View

In flagellar/ciliary motility what is the exact definition of a principal and reverse bend of beating flagellum/cilium?

This is something that has always got me confused. I come across so many papers but have not been able to find out on what basis does one basically categorise these two bends (principal and...

02 March 2017 3,972 0 View

What is the volume of a single Leishmania cell?

Can anybody suggest any references where the cell volume of Leishmania promastigotes has been measured?

04 May 2016 9,119 3 View

Mechanism of action of Dipyridamole?

I would like to know how effective a phosphodiesterase inhibitor is Dipyridamole? Does it inhibit both cAMP and cGMP specific PDEs? How effective is it? Any references will be helpful!

05 June 2015 7,921 0 View

Does anyone have experience with cloning and over expression of a large protein approx 450-500kDa?

I would like to know whether its possible to clone and overexpress a large protein of the size of approx 450-500 kDa without truncating any part of the protein?

08 September 2014 5,400 3 View

Can anybody suggest me any publications where the concentration of protein (say dynein) needed to perform an ATPase assay has been mentioned?

I would like to perform an ATPase assay on purified dynein, however I am totally clueless on what should be the concentration of the pure protein with which I perform the assay. Any suggestions or...

07 August 2014 5,990 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?

02 August 2024 9,518 2 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

How do I correct that wrong author's profile in my publication?

I am writing to request a correction regarding the affiliation of one of my co-authors, Donald Ho. Currently, the author's profile incorrectly lists an affiliation with The Hong Kong Polytechnic...

28 July 2024 6,824 0 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View