I would like to know how effective a phosphodiesterase inhibitor is Dipyridamole? Does it inhibit both cAMP and cGMP specific PDEs? How effective is it? Any references will be helpful!
I have dynein inhibitors, and want to prove that they inhibit the dyneins of Leishmania (my model organism of interest). I would like to perform an amino acid conservation analysis where I can...
10 November 2017 5,967 3 View
This is something that has always got me confused. I come across so many papers but have not been able to find out on what basis does one basically categorise these two bends (principal and...
02 March 2017 3,972 0 View
Can anybody suggest any references where the cell volume of Leishmania promastigotes has been measured?
04 May 2016 9,119 3 View
I came across this trypanosome specific phosphodiesterase inhibitor CpdA in few papers. However I could not find any company which sell the same. Any leads?
04 May 2015 552 1 View
I would like to know whether its possible to clone and overexpress a large protein of the size of approx 450-500 kDa without truncating any part of the protein?
08 September 2014 5,400 3 View
I would like to perform an ATPase assay on purified dynein, however I am totally clueless on what should be the concentration of the pure protein with which I perform the assay. Any suggestions or...
07 August 2014 5,990 2 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
There are three factors involved in the activity, the substrate acting as a substrate, the substrate acting as an inhibitor and another inhibitor for which you want to measure the Ki.
08 July 2024 4,988 1 View
Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...
12 June 2024 9,616 8 View
Antiplatelet Therapy: Mechanisms, Indications, Administration Can you explain the potential toxicity and overdosemanagement of commonly used antiplatelet drugs?
05 June 2024 6,447 1 View
I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...
31 May 2024 6,444 1 View
Can anyone interpret why the absorbance (DNS assay method) in alpha amylase assay get increased with increased standard (inhibitor) concentration? it should be decreased with increased standard or...
26 May 2024 4,753 2 View
I have a question regarding target miRNA expression after transfection with antisense miRNA (miRNA inhibitor). I understand that the expression of the target miRNA will increase after mimic...
20 May 2024 5,920 4 View
Estos articulos deben tener estas caracteristicas resumir los siguientes aspectos metodológicos: tipo de enfoque de investigación (cuantitativo, cualitativo o mixto), objetivo, hipótesis, muestra...
15 May 2024 820 0 View
I know that conventional wisdom dictates that protease inhibitors should be added to lysis buffers when extracting protein to prevent sample degradation. That said, why are such inhibitors...
14 May 2024 2,145 2 View
Dear all, One of the key points in TSAD (Time Series Anomaly Detection) when using a sliding window is the size of the window. For (quasi)-periodic time series, methods such as AutoPeriod,...
13 May 2024 920 3 View