How to perform amino acid conservation analysis of Dynein heavy chains??

I have dynein inhibitors, and want to prove that they inhibit the dyneins of Leishmania (my model organism of interest). I would like to perform an amino acid conservation analysis where I can...

10 November 2017 5,864 3 View

In flagellar/ciliary motility what is the exact definition of a principal and reverse bend of beating flagellum/cilium?

This is something that has always got me confused. I come across so many papers but have not been able to find out on what basis does one basically categorise these two bends (principal and...

02 March 2017 3,887 0 View

What is the volume of a single Leishmania cell?

Can anybody suggest any references where the cell volume of Leishmania promastigotes has been measured?

04 May 2016 9,011 3 View

From where can I purchase the phosphodiesterase inhibitor CpdA?

I came across this trypanosome specific phosphodiesterase inhibitor CpdA in few papers. However I could not find any company which sell the same. Any leads?

04 May 2015 413 1 View

Does anyone have experience with cloning and over expression of a large protein approx 450-500kDa?

08 September 2014 5,293 3 View

Can anybody suggest me any publications where the concentration of protein (say dynein) needed to perform an ATPase assay has been mentioned?

I would like to perform an ATPase assay on purified dynein, however I am totally clueless on what should be the concentration of the pure protein with which I perform the assay. Any suggestions or...

07 August 2014 5,898 2 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Has anybody administered MK-2206 (an orally active, allosteric inhibitor of AKT) intravenously to the mice?

We aim to use MK-2206 to inhibit AKT in mice. However, a large body of studies suggests that it should be administered orally. I was wondering if somebody could tell me that is it toxic for the...

07 February 2021 6,340 1 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View