For the PK study how to process the blood sample- plasma isolation and detection of drug using HPLC?

Jananishree Sathiyamoorthy @Jananishree-Sathiyamoorthy

28 September 2024 1 4K Report

For the PK study in an animal model, how to process the blood sample for plasma isolation and deproteinization for detecting the drug in HPLC

Abdelhak Maghchiche

To isolate plasma for HPLC analysis, centrifuge blood samples to separate plasma and then deproteinize using an organic solvent like acetonitrile or TCA. Finally, filter the supernatant and dilute if necessary before injecting it into the HPLC system for drug detection.

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