I am having some issues extraction total RNA from frozen samples of mouse skin, I used the "Total RNA Purification Kit" from Norgen. I got no RNA, so I am thinking this is cause I need a digestion solution to break down skin collagen matrix. I've been checking papers and the most of them use Trizol, has someone combine Trizol and the columns from the kit and gotten a good RNA quality?
My samples are going to be sequence for a transcriptomic proyect and I need them to have a really good quality.
Any advice is welcome :)
Yes, I used to extract with trizol and purify with Qiagen RNeasy columns and got very good quality RNA.
I usually homogenise my sample in Trizol and load directly to the DirectZol column kit by Zymo. Works very well!
I have already performed RNA extraction with Trizol and it worked very well, managing to detect housekeeping genes in the assay, but you could also use an RNA clean up kit to purify the RNA and obtain a purer material if you wish. Hope it help!
I homogenise sample in Trizol and extract using Zymo Directzol column kit.
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