at the time of calibration of the blank, OD is out of the spectrophotometer range, I got the error of setting the calibration, then what should I Do?
If OD readings are out of the spectrophotometer's range, dilute your samples or blank to bring them within measurable limits and ensure proper calibration of the instrument. Additionally, verify the freshness of reagents and consider alternative measurement methods if necessary.
If only the blank solution is out of range but not the sample, should I dilute just the blank or both the blank and the sample? Abdelhak Maghchiche
If the blank is out of range what is in it?? Is it phosphate that is producing a characteristic blue colour or something else with a wide absorption/scattering at 880 nm in the absence of molybdate? Be aware that some detergents are loaded with phosphate.
"Thank you for the insights! As per the molybdenum method, the blank in my case is Pikovskaya medium, which includes insoluble phosphate. I will verify whether any detergent, which could contain phosphate, has been accidentally added to the medium. Your explanation about the characteristic blue color from the molybdate-phosphate complex helps clarify potential interference. I will check the components carefully" Paul Milham
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