For performing transcardial perfusion in rats 0.1 M PBS at 7.4 pH is used. Is the 10x PBS has the molarity of 0.1 M ?.
Dear Omar Gonzalez-Ortega,
Hence 10x PBS should be diluted to 1 X to bring it to 0.1 M PBS?
Thank you
regards
Ankit Parikh
6.4University of Texas at Dallas
Does anyone know the procedure to prepare a 0.1M solution of PBS?
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Asked March 14, 2013
I have a 1X PBS solution with a 7.4 pH, and I need to prepare a 0.1M PBS solution from this for use in SEM sample fixation.
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SEM & TEM
Scanning Electron Microscopy
Sample Preparation
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Claudia Corbo
Claudia Corbo added an answer
March 15, 2013
Dear Ankit,
you can't do it.
Usually 1X PBS buffer is a solution with a phosphate buffer concentration of 0.01M (if you buy it from most of company); then you start from a dilute solution and you want a more concentrated one. At this point you should prepare it ex novo.
Regards,
Claudia
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Wolfgang H. Muss
Wolfgang H. Muss added an answer
May 27, 2016
Perhaps the anonymous Downvoter wants to donate some knowledge?
I am ready for being downvoted again...
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Wolfgang H. Muss
Wolfgang H. Muss added an answer
April 4, 2013
Dear Ankit, I recommend you to search e.g. Google for phrase [e.g. '0.1M solution of PBS' or '0.1M solution of PBS AND 1XPBS']. There are a lot of results (1.840), explaining what 1XPBS is and what not. Therefore usually (as commercially available): 10X PBS = 0.1M. Cave: there are several recipes out for making "PBS" (which only means: "Phosphate Buffered Saline", not indicating which "ingredients= salts" the solution will have incorporated (e. g. Na-phosphate(s), K-phosphate(s), NaCl, KCl, Na2HPO4, KH2PO4 etc.).
For making 0.1M PBS, search for < how to make "0.1 M PBS" > and you'll get a lot of answers too. This said just to be practical in advice (knowing that making NOT PBS but usually Na-NA-PO4-buffers like Millonig's or even Na-cacodylate buffer for preparation purposes in SEM and/or TEM preparations.
Best wishes and good luck, Wolfgang
------------Note / COMMENT / STATEMENT added 26th of February 2016------------ This my comment has been visited by the author for the first time since it was posted in March or April 2013 and was corrected Fri, 26th of February 2016 only regarding misspelling of 3 particular words.
Additionally I found out that my comment was downvoted 3 times without notification. I regret that the quality of my reply for 3 voters was unacceptable.
I thought first to delete the comment = Reply # 2 without substitution but found - in retrospective - that my original answer perhaps is / was a bit laborious but nothing was said wrongly.
The DOWNVOTERS*) (some of which might have been 'professional ones' as we / commenters personally are "hit" and exposed publicly sometimes for base motives, the latter might be understood by comparing the authors ResearchGate Score of 90 in 2013 with the recently achieved RG-Score of nearly 142 ) interestingly have overlooked the totally incorrect answer of Antonios M. (see Re#07 below) with regard to request of Zeravan M (Re#05):
from "Stock solution (0.1 M)" you can't make 1 M buffer solution.
I renounce to downvote that incorrect reply.
Regards, Wolfgang Muss
*) NB: from other RG-members to which I am bound I often am informed that their replies or questions are downvoted with obviously perfidious motives. I can't add any more specific to this since downvotes are anonymous.
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MSH Al-Furjan added an answer
May 8, 2013
Dear Ankit, I wish this link will help you.
http://www.thelabrat.com/protocols/reagents.shtml
http://www.thelabrat.com/protocols/3.sht
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Diego Baderna
Diego Baderna added an answer
September 3, 2014
Googling the same question I have found that traditional 1X PBS solutions which are commercially available correspond to 0.01 M. So you have to order the 10X solution.
Following the same theory you could dissolve 1 tablet of PBS from Sigma in 20 ml of deionized water and then filter with 0.22 um filter to sterilize the solution and to remove possible debris.
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Zeravan A. Mohammed
Zeravan A. Mohammed added an answer
December 5, 2014
hello every body could you please inform me how to make 10ml of 1M PBS solution ?
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Marco Nägele
Marco Nägele added an answer
February 10, 2015
rule of proportion?
by the way.. https://www.elabprotocols.com/viewer/?id=234
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Antonios Marmarinos
Antonios Marmarinos added an answer
December 14, 2015
You can make from scratch:
Stock solution (0.1 M)
Na2HPO4 (anhydrous): 12.0 g
NaH2PO4 · H20: 2.2 g
NaCl: 85.0 g
Distilled water: 1 liter
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Babak Rezaei
Babak Rezaei added an answer
February 25, 2016
Hi Ankit
It would be really simple to use PBS tablets to prepare PBS solutions with different concentrations. you can find more information in this link
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Wolfgang H. Muss
Wolfgang H. Muss added an answer
February 26, 2016
Dear Babak,
the URL you provided unfortunately deals with 1xPBS only. Ankit needs instead (as of his request!) 10xPBS. It might be that there are sold PBS-buffer tablets for 10XPBS too.
Again I would like to explicitely state that for use in "SEM-tissue sample fixation" and / or preparations 'PS'(= 'phosphate' buffers) as well as other biological buffers might be suited better than PBS (but -yes - one can!).
I am glad that Mozhdeh Ghani has upvoted your answer - and that I haven' downvoted your reply is confirmed by the fact that I definitely upvote your question which I couldn't do if I were 'your downvoter'.... Best wishes and regards, Wolfgang
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