for HA production, does it work if I use one integration plasmid which includes hasA gene from streptococus and the other plasmid includes tuaD ?is there any article which use this method?
I am not an expert in this field, but I am very interested and have researched to find an answer. I received some assistance from tlooto.com for this response. Could you please review the response below to see if it is correct?
Yes, using two separate plasmids with hasA and tuaD genes for hyaluronic acid (HA) production in Bacillus subtilis is feasible. The hasA gene from Streptococcus encodes hyaluronan synthase, while tuaD is involved in the biosynthesis pathway of the UDP-glucose precursor necessary for HA synthesis. Employing an integration plasmid for hasA and a separate plasmid for tuaD can work effectively, provided both plasmids are compatible and stable within the host cells. Studies have shown that co-expressing hasA and tuaD can enhance HA production significantly [1][3]. However, I could not find any specific article using this exact method of two separate plasmids [1][2][3].
Reference
[1] Widner, B., Behr, R., Dollen, S. V., Tang, M. R., Heu, T., Sloma, A., Sternberg, D., DeAngelis, P., Weigel, P., & Brown, S. D. M. (2005). Hyaluronic Acid Production in Bacillus subtilis. Applied and Environmental Microbiology, 71, 3747 - 3752.
[2] Saranraj, P. (2011). Production, Optimization And Spectroscopic Studies Of Hyaluronic Acid Extracted From Streptococcus pyogenes. International Journal of Pharmaceutical & Biological Archive, 2.
[3] Yang, M., Ferrari, E., Chen, E., & Henner, D. (1986). Identification of the pleiotropic sacQ gene of Bacillus subtilis. Journal of Bacteriology, 166, 113 - 119.
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