Hi. In determination of total flavonoid in foods , AlCl3 10% is needed, my question is that it should be prepare whit ethanol solouthion or methanol?
Sanket Rajendra Vakte
Weigh out the appropriate amount of anhydrous aluminum chloride (AlCl3) powder. The concentration you want is 10%, so for example, if you want to make 100 mL of a 10% AlCl3 solution, you would weigh out 10 grams of AlCl3. Add the weighed AlCl3 powder to a clean, dry glass container. Measure out the required volume of methanol. You can use anhydrous methanol for this purpose. In our example, if you want to make 100 mL of solution, add 90 mL of methanol to the container with the AlCl3. Seal the container and mix the contents thoroughly by gently swirling or stirring until the AlCl3 is completely dissolved in the methanol. Your 10% AlCl3 reagent solution is now ready for use. Store it in a tightly sealed container, away from light and moisture, as AlCl3 solutions can be sensitive to both.
In the determination of total flavonoids in foods using the aluminum chloride (AlCl3) method, it's common to prepare a reagent solution of AlCl3 in methanol. Methanol is often preferred over ethanol for this purpose due to its higher polarity and better solubility for certain flavonoid compounds.
Here's a general procedure for preparing an AlCl3 reagent solution for the determination of total flavonoids:
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Engineering
- Petroleum Engineering
- Oil
More Mehrzad Sayad's questions See All
Similar questions and discussions