Nazanin Mehrzad

3 Questions 7 Answers 0 Followers

Questions related from Nazanin Mehrzad

Bacillus subtilis 168 electroporation by low voltage?

Hi, I am trying to transform B.S 168 by electroporation and my vector s PHT43, can I use lower voltage for instance 1.5 kv with triple pulses instead (totally 15 ms)? I could not raise the...

25 December 2023 2,816 0 View

Expression of 2 genes is more efficient with one promoter or two ?

for heterologous expression in B.subtilis, I have used a triple promoter for expression of 2 genes in one vector , each gene has its own RBS, but there is only one triple promoter before gene 1,...

02 September 2023 8,560 11 View

Is triple promoter cloning in E.coli stable?

I am trying to clone 2 genes from bacillus subtilis within a triple strong promoter ( amyQ, amyL,amyE) in E.coli ( both EPI400 and JM109 )with PUC57 and pgD1662 vectors, but I failed for several...

01 January 1970 995 2 View