I had stored them in September 2012 after sub-culturing. When I checked them in May, 2013, there were about 50% viability, but they all died after few days when I passed them.
Hi,
What kind of freezing medium do you use?
Probably you just do but :
- while thawing you can try to be more delicate
- centrifuge the cels after thawing to eliminate DMSO
good luck
tommaso
Hi Tommaso, I am using 5% DMSO with RPMI medium. Now I got fresh THP-1cells and I did not have any trouble in culturing them and everything is just fine. Is this because I earlier kept them in Nitrogen vapors for too long? It happened with all cryopreserved vials. I really appreciate your response. Thank you
Hi Satvir,
i use 10%DMSO and 90% of FBS for freezing these cells, if you want you can try these medium and see if there are difference in the vitality of the cells after thawing.
have a nice day.
tommaso
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