For how long are the expressed mRNA stable in fungi?

More Pandit Ramesh's questions See All

How can I assemble paired end and shotgun sequencing data for whole genome assembly using SOAPdenovo2?

We have sequenced whole genome of fungi using 3K (250bp), 8K (250bp) and shotgun (450bp) library. How can we assemble these data up to scaffold level using SOAPdenovo2? Is there any good assembler?

08 September 2014 2,905 3 View

What is a good way to perform blast analysis of data against host pathogen interaction database? How can the HPI database be accessed?

I am working on Transcriptome sequencing of fungi. I would like to find pathogenecity related genes from fungi. HPI database provides this information, but I do not know how to make a blast or...

07 August 2013 1,811 5 View

I have sequenced a protease encoding gene from my fungi. How can I learn more about the coding regions and the presence of introns or exons?

I have sequenced about a 700 bp gene fragment of protease from genomic DNA. Now I would like to know the coding regions and whether it has any introns? Which software should I use?

01 February 2013 4,909 5 View

PLZ any one suggest me some free software through i can design specific primers for only Real Time PCR?

I would like to study expressed genes diring fungi infection.

11 December 2011 7,091 0 View

Hello friends i am working on Nematophagous fungi. Please anybudy give me name of book or paper or anything from which i can get classification of nem...

Generally it classification is based on morphlogy of fungi especially its traping structure and size and shape of spores.

11 December 2011 7,976 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Jay Siddharth

if u want to preserve mRNA use solutions like RNalater or similar, else freeze in liquid nitrogen.

Charissa de bekker

I did a lot of RNA extraction from fungal samples and I always stored them at -80C after snap freezing them in liquid nitrogen, when not using them directly. Though RNAlater works well for a lot of other tissue types, it, from my experience, reduces the efficiency of RNA extraction from fungal material.

Pandit Ramesh

Thank you. I also come to know that RNAlater not work properly for fungal samples is it true?