I would like to study expressed genes diring fungi infection.
We have sequenced whole genome of fungi using 3K (250bp), 8K (250bp) and shotgun (450bp) library. How can we assemble these data up to scaffold level using SOAPdenovo2? Is there any good assembler?
08 September 2014 2,905 3 View
I am working on Transcriptome sequencing of fungi. I would like to find pathogenecity related genes from fungi. HPI database provides this information, but I do not know how to make a blast or...
07 August 2013 1,811 5 View
I have to perform an induction experiment. If i preserve my induced fungal culture at -20C then could it affect the level of expressed genes? For how much time I can preserve it? Or do I have to...
01 February 2013 5,302 3 View
I have sequenced about a 700 bp gene fragment of protease from genomic DNA. Now I would like to know the coding regions and whether it has any introns? Which software should I use?
01 February 2013 4,909 5 View
Generally it classification is based on morphlogy of fungi especially its traping structure and size and shape of spores.
11 December 2011 7,976 0 View
I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together
28 July 2024 3,798 4 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...
22 July 2024 5,790 1 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
Give in detail with available literature and website links for submissions
27 June 2024 9,753 1 View
Hey everyone! I'm having difficulty marking the C4 complement factor in Western Blotting. Has anyone had contact with this antibody or made it in-house? I have already used Anti-C4β (D-12)...
17 June 2024 6,124 1 View
Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...
13 May 2024 3,061 11 View
I was curious as to why modern soybean half-seed protocols use 1/10x strength B5 salts during the co-cultivation step rather than full strength B5 salts? Also, are the B5 vitamins added at full...
07 May 2024 3,924 0 View
I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...
04 May 2024 6,463 0 View
How are these exploited in biotechnological applications?
07 April 2024 566 5 View
