How can I assemble paired end and shotgun sequencing data for whole genome assembly using SOAPdenovo2?

08 September 2014 2,828 3 View

What is a good way to perform blast analysis of data against host pathogen interaction database? How can the HPI database be accessed?

I am working on Transcriptome sequencing of fungi. I would like to find pathogenecity related genes from fungi. HPI database provides this information, but I do not know how to make a blast or...

07 August 2013 1,738 5 View

For how long are the expressed mRNA stable in fungi?

I have to perform an induction experiment. If i preserve my induced fungal culture at -20C then could it affect the level of expressed genes? For how much time I can preserve it? Or do I have to...

01 February 2013 5,215 3 View

I have sequenced a protease encoding gene from my fungi. How can I learn more about the coding regions and the presence of introns or exons?

I have sequenced about a 700 bp gene fragment of protease from genomic DNA. Now I would like to know the coding regions and whether it has any introns? Which software should I use?

01 February 2013 4,810 5 View

PLZ any one suggest me some free software through i can design specific primers for only Real Time PCR?

I would like to study expressed genes diring fungi infection.

11 December 2011 7,011 0 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

Why negative controls also showing the specific bands of promotor and other regions (GUS, GFP etc.)?

Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...

06 January 2021 8,465 99 View