For field screening of rice genotypes against brown plant hopper, which of the two scales is standard scale for evaluation?

More Rajan Kamboj's questions See All

I am trying to resolve immune cytokines. Which is the right percentage of SDS resolving gel?

I wish to resolve IL-6 (mol wt= 21-26KDa), TNF- alpha (17.3-17.4 KDa), GMF-beta (16.7 KDa), GAPDH (36kDa) and PDL1 (40-70 KDa). I think 12.5% gel is good to go. Is it advisable or should I go for...

25 April 2024 9,798 0 View

How do i calculate cut off values in ELISA?

I am running ELISA for different biomarkers against meningitis. I need to know how to calculate cutoff values for it in ELISA. There is no negative control or positive control for these kits,...

07 April 2024 3,079 1 View

Need to justify choice of two deep learning models CNN and sae compared to other models in data analysis ?

I have selected two deep learning models CNN and sae for data analysis of a 1 d digitized data set. I need to justify choice of these two dl models in comparison to other dl and standard ml...

18 January 2024 6,800 1 View

Need to justify choice of two deep learning models CNN and sae compared to other models in data analysis ?

I have used two dl models CNN and sae for data analysis of 1 d digitized data set. I need to justify use of these two models in comparison to other dl and other models. I am using ga to optimize...

18 January 2024 9,816 0 View

Which primers should I use for random amplification of bacteria from CSF sample?

I am working on 16s rRNA gene sequencing. So for that I should do PCR with random primers. Suggest me with a primer that I should use, also the which universal buffer the ti can use and if...

14 December 2023 6,288 0 View

(How) Is it feasible to use a questionnaire (of closed ended questions) to explore the lived experiences of participants in a phenomenological study?

Is it usual to use the closed ended questions in a Google sheet before carrying out semi-structured interviews for collecting data in a phenomenological study? If it is possible how can it be...

08 December 2023 2,290 7 View

Can anyone suggests a free software for finding particle size ?

For the measurement of particle size from XRD TEM SEM etc

12 November 2023 8,821 2 View

Is there any alternate way to avoid perfusion using a perfusion buffer for isolation of liver cells for primary culture?

Currently, I am working on mouse or rat models' primary culture of liver cells (particularly hepatocytes). In most of the previous research, I found the protocol stressing the perfusion of the...

10 October 2023 3,685 0 View

Can anybody provide me a reference about, TAHARA task which is based on the flow solver created by Prof. Yusuke Tahara of Osaka Prefecture University?

Required to understand how to solve potential flow around a ship hull.

29 September 2023 6,949 0 View

What percentage of scientific papers get more than 200 citations?

I know that many papers get 0 citations. I have read that 1.8% of papers get more than 100 citations. How about 200? What percentage of papers get 200 or 500 citations?

10 September 2023 5,973 1 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Hello dear researchers. I would like to know what is a normalized mass in an EDX analysis?

EDX analisys

31 July 2024 5,947 2 View

Gas chromatography RT detection?

I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...

29 July 2024 8,034 4 View

MAGeCK Plotting Error: Logarithmic Axis Must Have Positive Limits?

I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...

28 July 2024 9,811 1 View

Screening of EPS producer LAB?

if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...

26 July 2024 6,606 0 View

Could international experts in media and journalism, AI, communications and international communication share this conference link?

"Integration of Mass Media and Communication into the System of International Relations: Place, Role, Actuality and Prospects: Modern Media Issues 2024" Website: https://immcir.alnoor.edu.iq/

23 July 2024 3,139 6 View

Why methanol and sulphuric acid used in the analysis of polyhydroxyalkanoates (PHA) by GC-MS?

Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?

22 July 2024 1,210 2 View