We are working on the structural biology of FEN1 complexes and mutants, but would like to test activities in vivo in fission or budding yeast or other model systems. Which cell systems make sense besides mammalian and human cells?
I think a good system would be any that employ GAL-HO to make a site specific DSB. For example, JKM179(moore and Haber,1996) , which is commonly used to asses non-homolgous end joining ,YMV80(Vaze et al,2002) which repairs by single strand annealing and YJK17(Kim and haber 2009) which repairs by inter chromosomal gene conversion.
Do you have ideas for looking more directly at replication fork related activities and defects?
Im not exactly sure how to look at that, but this paper might describe a useful system?
http://www.nature.com/nsmb/journal/v16/n2/abs/nsmb.1544.html
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