24 August 2016 1 4K Report

Looking for anyone with experience in assessment of differences between linear construct design versus plasmid based, STARR-Seq method...

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690393/?

More Kenneth Day's questions See All
What are the challenges or short falls with the ISO 45001?

In 2018 the OHSAS 18001 was improved and progressed into ISO 45001. This was characterized by one or two short falls. I am now reviewing the ISO 45001, with the aim of looking at the entire...

18 July 2024 9,489 0 View

What are the estimation methods and their properties, and the edge they have over others, especially the maximum likelihood estimator?

what are the estimation methods and their properties, and the edge they have over others, especially the maximum likelihood estimator? what is multinomial logistic regression and its properties...

03 May 2024 1,261 1 View

How can healthcare systems collaborate to enhance the comprehensiveness and utility of regional antibiograms in guiding antibiotic therapy?

This question prompts exploration into collaborative approaches to improve the quality and relevance of regional antibiograms. This encourages critical thinking about potential strategies for...

11 April 2024 2,770 0 View

Please, I need a suggestion on the most recent and best(suitable )PHREEQC database for surface-groundwater mixing/modeling?

The parameter of interest includes; Major ions and TROCs.

01 April 2024 8,466 1 View

Genotyping plants for overexpression insertion?

I have plants (L. japonicus) that have been transformed with an overexpression plasmid. How can I know that these plants are homozygous for the insertion?

24 March 2024 8,533 2 View

Are there any guidelines for using CHAT GPT in your research paper title?

I always take the longest time thinking about titles and writing abstracts, so I think it might be a good use. But I wonder if journals should include this in their guidelines, or else, it's not a...

12 February 2024 7,730 1 View

Is there anything like "indigenous Multilingual Pronunciation"?

In a multilingual setting, Is there anything like "indigenous Multilingual Pronunciation"?

30 January 2024 8,287 1 View

You requested I verify I am elgible to post, but the link did not work. How do we fix this?

My name is Kenneth Neil Swartz, and I have numerous articles already posted on Research Gate wit a co-author. Below are some examples. Please approve my account so I can post a paper as a...

30 January 2024 9,593 0 View

Evaluation of an HSE management system ?

How is an HSE management system evaluated, to trigger a sustainable workplace?

24 January 2024 2,121 2 View

Why do I get more than 100% in my upper plateau when determining IC50?

I am working on an inhibition assay. I included 10-12 concentrations for the assay to account for the upper and lower plateaus of the sigmoid curve. To make the calculation of IC50 easier, I used...

27 August 2023 2,986 0 View

Similar questions and discussions
Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

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