Kenneth Day

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Questions related from Kenneth Day

For an in vitro enhancer screen assay, would you use linear construct design or plasmid based, STARR-Seq method?

Looking for anyone with experience in assessment of differences between linear construct design versus plasmid based, STARR-Seq method... https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690393/?

24 August 2016 3,825 1 View

Does anyone have experience with immunoprecipitation or Dynabead pulldown of large TdT end labeled(biotinylated) restriction fragments (>10kb)?

Genomic DNA is digested with a restriction enzyme, end labeled with biotin-dNTP with TdT, and then immunoprecipitated with Protein G Dynabeads coated with anti-biotin antibody.

05 December 2014 8,176 14 View