Kenneth Day

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Questions related from Kenneth Day

For an in vitro enhancer screen assay, would you use linear construct design or plasmid based, STARR-Seq method?

Looking for anyone with experience in assessment of differences between linear construct design versus plasmid based, STARR-Seq method... https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690393/?

24 August 2016 3,841 1 View

Does anyone have evidence for M-270 streptavidin-Dynabeads being more efficient for capture of longer DNA fragments compared to MyOne C1 beads?

Streptavidin-Dynabeads are typically used for in solution capture with biotinylated probe. Life Tech states that due to steric hindrance, MyOne beads are not efficient for pulldown of large DNA...

09 January 2016 1,119 0 View

Does anyone have experience with immunoprecipitation or Dynabead pulldown of large TdT end labeled(biotinylated) restriction fragments (>10kb)?

Genomic DNA is digested with a restriction enzyme, end labeled with biotin-dNTP with TdT, and then immunoprecipitated with Protein G Dynabeads coated with anti-biotin antibody.

05 December 2014 8,197 14 View