If so, how do I correct it and make it more diagonal? Two cell lines (cancer cells) show FSC-A and H like this. I tried to play around with voltages but could not get a more "diagonal" scatter plot for area vs. height. Any help is appreciated.
Hi Pavan,
the data looks fine for me. It seems you do not have doublets. Just mention that cell line 2 seems to have a more heterogeneous cell population in terms of size: higher FSC-A value, so bigger cells.
In any case, if you want to see the data more "diagonal", you can just reduce the Y-axis range; i.e. make FSC-H axis to measure until 150K a.u. maximum. That way, it will appear as diagonal.
Maybe that way you can separate some doublets (some points that have FSC-A > FSC-H, but I think it´s very subtle).
Good luck
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