Are machine learning (deep learning) and AI threats to the job security and employment opportunity of technical staff and even senior executives?

The most common example is the GOOGLE lens application. The experience and decision making ability acquired by the human beings is limited as compared to the BIG DATA that an intelligent machine...

31 May 2020 2,517 3 View

How to correct invalid atom type error in gromacs?

27 April 2020 1,318 3 View

How to fix lincs warning in free energy calculation?

Dear Researcher I am having trouble during free energy calculation in gromacs as below:- I ran an MD simulation for 3ns and from the last coordinates started a free energy calculation for 3ns. My...

24 March 2020 5,311 9 View

When values given in Median and IQR, is it possible to convert them into Mean with std dev?

I am doing a meta-analysis on GDM, I have values for Parity and Gravida in the median with IQR where the N is known. I want to convert them in mean with std dev.

29 February 2020 8,809 2 View

I want to calculate Odds Ratio for meta analysis?

I have twelve articles for my MA for Gestational diabetes Mellitus. I want to calculate OR for Age>25, BMI>25,Multiparity >2 ,Primigravida. The problem is for all the risk factors(age,...

23 February 2020 9,313 5 View

How to fix Residue mapping error for an amino acid in gromacs?

Dear Gromacs users I want to do MD simulation for my protein but when I run pdb2gmx command I am facing this error:- WARNING: WARNING: Residue 197 named PRO of a molecule in the input file was...

12 November 2019 5,425 3 View

Why editconf removes ligand during box formation in gromacs?

Hi Gromacs users I am trying to set a protein-ligand system in gromacs for MD simulation. I took topology and gro file from an external source. Now I combine the gro file of ligand and protein...

09 November 2019 2,785 8 View

How to use Ponceau for dot blot?

01 November 2019 1,193 3 View

I have some problems with Tris-Tricine SDS PAGE. Any one knows about it ?

Hey everyone! As I run my hydrolyzed samples in the Tris-Tricine buffer system, the bands were disappeared as the samples run into the gel. Lane 1 = Marker (10 - 180KDa) Lane 2 = BSA...

20 October 2019 6,412 3 View

Hey everyone ! I need to extract protein from Sea cucumber Anyone have protocols ?

Hello !!! I have tried many different methods for protein extraction from Sea cucumber (Apostichopus japonicus) i.e. Acid treatment, Alkaline methods, and TCA precipitation along with ethanol,...

21 September 2019 3,246 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View